Intermediate

Part:BBa_K117007:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-08)

colE7ImmE7-double terminator BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Col E7 and Immunity

Colicin E7 is known to degrade chromosomal and plasmid DNA. The rate at which the degradation occurs was found to be directly proportional to E7 concentration.


Source

Part BBa_K117001 was constructed by NTU iGEM 2008 team. Part BBa_B0015 was obtained from the Registry

References

Folia Microbiol. 35, 348-352 (1990). Colicins E7 and E8 Degrade DNA. in Sensitive Bacteria. J. MARDA', J. MARDA Jr. h and Z. VRBICKA

Structure. Volume 7, Issue 1, 15 January 1999, Pages 91-102 T.P. Ko, C. C. Liao, W. Y. Ku, K. F. Chak and H. S. Yuan, The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein